MicroRNAs(miRNAs) are naturally occurring non-coding RNAs of 18-25 nt, which can bind to target RNAs based on sequence complementarity and direct post-transcriptional regulation of gene expression and regulate the stability or translational efficiency of target mRNAs. Numerous studies suggest that miRNA-mediated silencing may play an important role in development and disease, implying that miRNAs can serve as valuable biomarkers for diagnostic approaches.
It was first discovered in 1993 by genetic screens. The following discovery of a second miRNA and its conserved temporal expression patterns reveals that miRNAs is conserved among eukaryotes. Their diversity has been confirmed using bioinformatics and subsequent cloning. The analysis of miRNA knockout mice uncovered the physiological importance of individual miRNAs in mammals.
miRNAs belongs to the large miRNA genes family which has great diversity in genomic organization. Examination of mammalian miRNAs revealed that about one-third are expressed from introns of known protein-coding genes, with the remainder found distant from previously annotated genes. Whereas intronic miRNAs can be transcribed simultaneously with their host genes, intergenic miRNAs can be expressed from independent transcription units. They are frequently found as polycistronic clusters that are coordinately expressed. Although RNA Pol II transcribes the majority of miRNAs, RNA Pol III-transcribed miRNAs have been found.
Creative Biogene is leading in microRNA technologies and can offer full lines of miRNA related products incuding microRNA discovery, expression profiling and functional analysis.
Quantification of miRNA by real-time PCR is a powerful tool for study of miRNA. Real-time PCR is used to validate the expression of miRNAs discovered during high throughput arrays. It is also a useful tool to discover new miRNAs and to study the expression of individual miRNAs.
Creative Biogene provides clones for over-expression of miRNA, miRNA mimics and inhibitors of your choice. All known human, mouse and rat miRNAs in the current miRBase is covered.
Creative Biogene offers genome-wide human, mouse and rat miRNA 3'UTR target clones in mammalian expression vectors. The 3'UTR plasmids provide a convenient solution for quantitative assessment of the inhibitory effect between miRNAs and their potential target genes. Creative Biogene also provides 3'UTR Virus Particles for delivery of 3' UTR into a wide range of cell types including difficult-to-transfect cells.
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